Ozone high dose therapy (OHT) improves mitochondrial bioenergetics in peripheral blood mononuclear cells
König B, Lahodny J. Translational Medicine Communications, 2022.
- Study type
- Case series
- People studied
- 6 people
- Published
- 2022, Translational Medicine Communications
- Identifiers
- PMID 35880042, DOI 10.1186/s41231-022-00123-7
What the authors reported
In 6 patients given two 10-pass ozone high-dose therapy (OHT) sessions within a week, a laboratory bioenergetic health index in blood cells improved, driven mainly by increased mitochondrial reserve capacity.
What limits this result
Six patients, no control group; a laboratory biomarker rather than a symptom or clinical outcome, co-authored by the technique's own developer. The abstract does not state a specific cumulative ozone dose in micrograms.
How ozone acts on blood and cells, how blood filters work, the laboratory and device-development studies behind the EBOO circuit, and studies that measure markers in the blood rather than how patients fare. None of these shows whether EBO2 helps patients. One study rarely settles a medical question; our guides weigh each paper against the rest of the evidence.
How the study was done
- Design
- Pilot study of six consecutive patients given preventive ozone high-dose therapy (OHT)
- Setting
- Private Department of Gynecology and General Medicine (Dr. Johann Lahodny), St. Pölten, Austria
- Read from
- Read from the full text of the paper. Read on September 28, 2026.
Study arms
| Group | Participants | What they received |
|---|---|---|
| Six patients given preventive 10-pass OHT | 6 | OHT for preventive purposes, twice within one week |
Source: Abstract on PubMed
Procedure as reported
- Ozone concentration
- 70 μg/mL
Oxygen ozone gas mixture, 200 mL, is generated at an ozone concentration of 70 μg/mL
- Blood volume
- 200 mL of patient’s blood withdrawn per pass
200 mL of patient’s blood is withdrawn from a peripheral vein into a vacuum flask containing of 7500 units of heparin
- Session length
- Approximately one hour for one L1 (10 passes)
- Sessions
- 2 OHT applications over a period of 1 week
- Anticoagulant
- 7500 units of heparin (in the vacuum flask)
In the paper's own units, not converted.
What it measured
- Bioenergetic health index (BHI) in peripheral blood mononuclear cells
- Improves significantly after just 2 OHT applications over a period of 1 week
- Reserve capacity and maximum respiration of the mitochondria
- Significant increase; the overall BHI improvement is based primarily on it
- Non-mitochondrial oxygen consumption
- Increased; the paper says this increase has a negative impact on the BHI value
- BHI comparisons between the three sampling times (V, N, NN as defined in the Fig. 1 caption)
- P-values: 0,045 (NN versus V); 0,198 (N versus V); 0,079 (N versus NN)
Sources: Abstract on PubMed, Full text on Europe PMC
Safety as reported
No adverse events are described for the six patients. The introduction says of major autohemotherapy generally that it has been carried out “without side-effects and with therapeutic results, albeit poorly documented”
Source: Full text on PubMed Central
Follow-up
About one week: blood taken before treatment, immediately after the first treatment, and after the second treatment one week laterDuring this pilot study the blood which was routinely taken before treatment, immediately after the first treatment, and after the second treatment one week later was used for analysis of mitochondrial functions as well.
Source: Full text on PubMed Central
Funding and conflicts
Competing interests: JL has pioneered the 10-pass OHT method but does not hold a patent on it; JL uses the method at his private clinic and is invited to lectures worldwide
Source: Full text on PubMed Central
Where we cite this study
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